Rarefaction Helper

v1.0 — web edition

Upload a feature table or QIIME2 artifact to visualize sample read depth distributions, explore how many samples are retained at any rarefaction depth, and compute rarefaction curves — with data-driven depth suggestions and honest caveats about context

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Drop a feature table or QIIME2 artifact here

or browse · .qzv (table summary) · .qza (feature table) · .tsv / .biom (exported table) · .tsv / .csv (per-sample depths)

Supported inputs:

QIIME2 table summary .qzv — from qiime feature-table summarize. Extracts per-sample depths; enables depth distribution, retention analysis, and suggestions.
QIIME2 feature table .qza — parsed if BIOM JSON format (v1.0); HDF5 tables (v2.1, most QIIME2 outputs) cannot be read in-browser — see the note below.
Exported feature table .tsv — from biom convert -i table.biom -o table.tsv --to-tsv. Enables full rarefaction curve computation.
BIOM JSON .biom — BIOM v1.0 JSON format feature tables.
Per-sample depth file — any two-column TSV/CSV with sample IDs and read counts.

HDF5 note: Most QIIME2 feature tables use HDF5 BIOM v2.1, which requires native libraries to parse. Upload your table-summary .qzv for depth analysis, or export the table first: qiime tools export --input-path table.qza --output-path exported/ && biom convert -i exported/feature-table.biom -o feature-table.tsv --to-tsv

All processing happens entirely in-browser — no data is uploaded or stored.